Results from Interseeding Cover Crops into Corn or Soybean

Homemade interseeder
Figure 1. In 2019 in York County (left), the grower built his own interseeder and tested a nitrogen mix (4 lb/ac crimson clover, 3 lb/ac red clover, 2 lb/ac yellow sweet clover, 4 lb/ac Winterhawk annual ryegrass, 1.5 lb/ac impact forage collards, 1.5 lb/ac Trophy rapeseed) vs. diversity mix (2 lb/ac red clover, 2.5 lb/ac Hubam white seed clover, 4 lb/ac Winterhawk annual ryegrass, 1 lb/ac purple top turnip, 3 lb/ac golden flax, 0.5 lb/ac phacelia Angelia, 0.5 lb/ac chicory) interseeded at V5-V6 corn vs. check treatment. Seward County (right), a Hagie was used to broadcast interseed 10 lb/ac red clover and 5 lb/ac buckwheat into V6 corn vs. check treatment.

Results from Interseeding Cover Crops into Corn or Soybean


Interest in interseeding cover crops into living corn and soybean has increased in recent years. In a companion article, we share what we’ve learned thus far from interseeding cover crops into living corn and soybean. This article will focus on the results of studies conducted via Nebraska On-Farm Research and via Soybean Management Field Days.

Nebraska On-Farm Research Studies: Interseeding into Corn

The Nebraska On-Farm Research studies were conducted at two locations in York and Seward counties (interseeded into V5-V6 corn) in 2019 (Figure 1) and at six locations in Seward, York, Clay and Hamilton counties (interseeded into V4 corn) in 2020 (Table 1). In 2020, four of the six locations compared an interseeded cover crop to a check treatment of no cover crop. One location compared two corn populations (27,000 vs. 31,000 seeds/ac) to determine corn yield and cover crop biomass impacts. One location compared using only the middle drill unit to interseed the cover crop vs. using all three drill units between the rows to determine any differences in cover crop biomass.

Table 1. Sites, location, year, replications, cover crop mixtures, interseeding dates, row direction and irrigation status for six sites evaluating cover crop interseeding.
IDReport IDCountyCover Crop MixInterseeding DateRow DirectionIrrigation
2020-1 0145159202001 Seward Diversity Mix 6/1/20 East-West SDI
2020-2 0580035202001 Clay Nitrogen Mix 6/3/20 North-South Pivot
2020-3 0916185202002 York Custom Mix 6/1/20 North-South Pivot
2020-4 0618159202001 Seward Diversity Mix 6/8/20 East-West Pivot
2020-5 0073081202001 Hamilton Diversity Mix 6/3/20 East-West Pivot
2020-6 0918159202001 Seward Diversity Mix 6/8/20 North-South Pivot

Results: Biomass

Interseeded mixes chart
Figure 2. Mixes interseeded into V4 corn in 2020.

In 2019, the cover crop at the Seward County location emerged and then died, we hypothesize, due to reactivation of Group 27 herbicide. Thus, no biomass samples were taken. At the York County location, cover crop biomass sampled prior to the first hard freeze ranged from 97-220 lbs/ac. It was good to see successful establishment at both locations and that cover crop growth occurred at one of them.

In 2020, cover crop biomass samples were taken at the end of September 2020 by taking three 30”x30” or 36”x36” squares for each treatment (depending on row spacing). The samples were sorted in the field into weeds, interseeded forbs/legumes, and interseeded grasses and placed in separate paper bags. Samples were weighed and dried. We wanted to compare any differences in weed biomass between the check and interseeded treatments.

Only at site 2020-3 was there a difference in weed biomass between the check and interseeded cover crop treatments. The weed biomass at this site was greater for the interseeded treatment as the check was cultivated for weed control, resulting in lower weed pressure (Figure 3). There was 45% greensnap at site 2020-5 due to a July 9, 2020 windstorm.

The open canopy resulted in greater weed and cover crop biomass. Total cover crop biomass accumulated varied by site and ranged from a minimum of 277 lb/ac at site 2020-2 to a maximum of 3818 lb/ac at site 2020-4. It should be noted that cover crop biomass continued to accumulate until the first hard freeze; these numbers don’t account for any additional accumulation.


Cover crop biomass
(Photo by Jenny Rees)

Soil samples were taken in early September to determine any nutrient and biological differences between the check treatments and the interseeded ones. Haney and Phospholipid Fatty Acid (PLFA) tests were taken to determine beginning soil health and microbial parameters, but to aid in cost savings, the data was not analyzed by replication. The sites were not combined for statistical analysis for the soil nutrient levels; however, results from individual sites can be viewed on pages 136-147 of the 2020 Nebraska On-Farm Research results book.

In general, the trend observed was lower soil nitrate and potassium in the interseeded treatments compared to the check strips. We observed the cowpeas, hairy vetch, sweetclover and forage soybean fixing nitrogen in the growing season of 2020. The hairy vetch and red clover that survived the winter were fixing nitrogen in May 2021. We plan to take soil samples in the spring of 2021 and corn tissue samples in 2021 to determine any additional nitrogen benefit that could be contributed to the legume cover crops.


In 2019, there were no yield differences between the corn in the check treatments (241 and 258 bu/ac) vs. cover crop interseeded treatments (241 (N mix), 243 (diversity mix), and 256 bu/ac) at the York and Seward locations, respectively. Net return was less for the interseeded cover crop treatments vs. the check. The 2019 results and additional details can be viewed at this link.

In 2020, a July 9 windstorm impacted the area of the state where these studies occurred. Hybrids varied in their development stages and varied in their response (regarding bending or breaking). Both responses impacted yields. Yield from the studies were analyzed as a large group by comparing the check and interseeded treatments (Figure 3). There was no interaction of site and treatment; therefore, treatments are examined across all sites.

Across all the sites, corn yield for the check averaged 214 bu/ac while corn yield for the interseeded treatment yielded 209 bu/ac (p-value=0.001). At four of the six sites, yield was significantly lower where the interseeded cover crop was used (sites 2020-2, 2020-3, 2020-5, and 2020-6). At the remaining two sites (2020-1 and 2020-4) yield was not different between treatments. Net return for the corn where the cover crop was interseeded was less at five of the six locations (Figure 3). Net return includes the yield and price of the corn crop and cost of cover crop seed and application. In the future, any benefits observed such as reduced nitrogen, herbicide cost, etc. due to the cover crop could be factored in the net return calculation.

Other than the York County location (two years), all the location data is based on one year of research. These studies will continue in the same fields and strips for at least three years, so it will be interesting to watch for any changes in soil biological and physical properties over time as well. Visually, in the field where the center drill unit vs. three were used, it appeared that the one drill unit had more biomass. Statistically, it ended up the same as the three drill units for total cover crop biomass. At the York location where cover crops were interseeded into two corn populations, there were no yield differences between the corn populations; however, both yielded less than the check treatment.

A special thanks to all the growers working with us on these interseeding cover crop studies and to The Nature Conservancy, Upper Big Blue NRD, NRCS, and Kellogg’s for their partnership with Nebraska Extension on this effort.

Biomass accumulation
Figure 3. Weed and interseeded cover crop biomass accumulation, yield, and net return from six locations in 2020. Points are means and bars represent one standard deviation from the mean.

* Means statistically significant at the 90% confidence level.

Interseeding soybeans
Figure 4. Hiniker interseeding into V3 soybean.
Interseeding soybeans
Interseeding soybeans

Soybean Management Field Day Studies: Interseeding into Soybean

Studies were established at all 2020 Soybean Management Field Day locations (Arlington, Shelby, Elgin, Hildreth). A cover crop mix of annual rygrass (2lb/a) and winter wheat (10 lb/a) was drilled interseeded using a Hiniker cover crop drill interseeder at soybean V3 growth stage (Figure 4). Standard height and short-stature soybean varieties planted at 100,000 seeds/acre were used. Two herbicide programs (Preemergence followed by Postemergence (PRE fb POST) and POST only) were tested. The PRE herbicide application was Valor at 3 oz/A applied at planting and the POST application was Roundup at 32 fl oz./A plus applied prior to cover crop interseeding. Soybean planting and cover crop interseeding dates are listed in Table 2. Data collected includes cover crop biomass, weed suppression following POST application, and soybean grain yield.  

Table 2. Planting and application collection dates at each of the Soybean Management Field Day sites in 2020.
SiteCover Crop InterseededSoybean PlantedSoybean Variety Herbicide
StandardShort PREPOST
Arlington June 24 May 11 2.3 3.3 May 11 June 24
Shelby June 24 May 18 2.7 3.7 May 18 June 24
Elgin June 25 May 19 2.0 3.0 May 19 June 25
Hildreth June 25 May 12 2.5 3.6 May 12 June 25

Data Collection

Yield: Soybean yields were taken using a small plot combine by harvesting the center two rows of each plot. Alleys were cut just prior to harvest and recorded to determine total plot length. All yields were adjusted to 13% moisture prior to the statistical analysis.

Statistical analysis. The experimental data was analyzed to evaluate cover crop effects on yield. Significant differences are based on a probability of a = 0.05.

Interseeded cover crop under canopy
Figure 5. Interseeded Cover crop under soybean canopy on Aug. 4, 2020.
Interseeded cover crop under canopy
Interseeded cover crop under canopy


Cover Crop biomass: It was observed that wheat cover crop emergence following planting, but all cover crop treatments died under soybean canopy cover by the end of the season.

Yield: There was a cover crop by herbicide interaction for the Elgin location where preemergence plus postemergence herbicide treatment resulted in higher yields with the wheat and annual rye cover crop mix compared to the postemergence only herbicide treatment (Figure 8). There were no other yield differences for any of the other study locations (Figures 6, 7 and 9).  

Interseeded cover crop under canopy
Figure 6. Soybean grain yield (bu/acre) by cover crop/no cover crop treatment, soybean variety, and herbicide treatments at the Arlington location.
Interseeded cover crop under canopy
Figure 7. Soybean grain yield (bu/acre) by cover crop/no cover crop treatment, soybean variety, and herbicide treatments at the Hildreth location.
Interseeded cover crop under canopy
Figure 8. Soybean grain yield (bu/acre) by cover crop/no cover crop treatment, soybean variety, and herbicide treatments at the Elgin location.


Soybean grain yield chart
Figure 9. Soybean grain yield (bu/acre) by cover crop/no cover crop treatment, soybean variety, and herbicide treatments at the Shelby location.

For interseeded cover crops, light availability mid-season is often the most limiting factor affecting growth and survival. The study selected grass cover crops that have some shade tolerance, reduced soybean population from 130,000 seed per acre to 100,000 seed per acre and tested a shorter soybean variety to determine what effect this may have on cover crop survival. At the conclusion of this study, it was determined that these did not have a significant enough effect on light penetrating through the soybean canopy mid-season to result in season-long cover crop growth. In addition, there were almost no treatment effects on soybean yield.

A follow-up to this study has been planned for future SMFDs to evaluate the timing of cover crop interseeding/planting (fall planted, at soybean planting, and V3 soybean growth stage), soybean variety, (determinate vs. indeterminant), and soybean plant population. We will also test the use of banded compared to broadcast preemerge herbicide application in combination with cover crop treatments to evaluate the effects on weed suppression. We have concluded that additional investigation and testing is needed to determine if cover crop interseeding in soybean is a viable option for Nebraska soybean growers.  

*Acknowledgements: The authors would like to acknowledge the following for helping with the on-farm research studies: Our colleagues Melissa Bartels and Michael Sindelar, who aided in biomass sampling, and graduate students Morgan Grabau and Alyssa Kuhn, who helped with weighing and drying biomass samples.

Helpful Resources

Nebraska On-Farm Research Virtual Field Day Interseeding Videos:

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